We are looking for a PhD candidate to study the molecular and cellular control of the barrier function of endothelial cells. Specifically, we want to study the dynamics of the cellular junctions and the connected cytoskeleton using super resolution microscopy.
You will be part of the team of Molecular Cytology at the Swammerdam Institute for Life Sciences in the Faculty of Science at the University of Amsterdam and have access to the van Leeuwenhoek Centre for Advanced Microscopy. The project is part of the Vascular Immunology Consortium, an ambitious interdisciplinary research initiative supported by the University of Amsterdam. This consortium bridges the Faculties of Life Sciences and Medicine, fostering a highly collaborative and integrative research environment. The overarching objective of the consortium is to elucidate the complex mechanisms by which the vascular system regulates the human immune response, with a particular focus on how vascular integrity and function are modulated under inflammatory conditions, including leukocyte extravasation and the biophysical stresses imposed by hypertension and blood flow.
We are looking for a PhD candidate to study the molecular and cellular control of the barrier function of endothelial cells. Specifically, we want to study the dynamics of the cellular junctions and the connected cytoskeleton using super resolution microscopy.
You will be part of the team of Molecular Cytology at the Swammerdam Institute for Life Sciences in the Faculty of Science at the University of Amsterdam and have access to the van Leeuwenhoek Centre for Advanced Microscopy. The project is part of the Vascular Immunology Consortium, an ambitious interdisciplinary research initiative supported by the University of Amsterdam. This consortium bridges the Faculties of Life Sciences and Medicine, fostering a highly collaborative and integrative research environment. The overarching objective of the consortium is to elucidate the complex mechanisms by which the vascular system regulates the human immune response, with a particular focus on how vascular integrity and function are modulated under inflammatory conditions, including leukocyte extravasation and the biophysical stresses imposed by hypertension and blood flow.
The central aim for this project is to understand the molecular and cellular control of the barrier function of endothelial cells. In forming a monolayer, these cells partially overlap and form a tight barrier resisting sheer force and blood pressure. At the molecular scale, vascular endothelial (VE) cadherin and other proteins form a junctional protein complex (adherence junction), which tightly holds plasma membranes together of adjacent endothelial cells in a proximity of 20 nm. Intracellularly, these cell junctions are connected to the actin cytoskeleton via (α,β and p120) catenin. A variety of signalling cascades are connected to the regulation of the barrier integrity and cytoskeletal remodelling (including VEGFR and small GTPases).
This project focuses on the dynamics of the adherence junction and the connected cytoskeleton in relation to (local) pertubation by optogenetics, drugs and leukocyte transendothelial migration using super resolution microscopy. We will use a novel tau-STED microscope, which can achieve 50-70 nm optical resolution in live cells. By exploiting time-resolved information (ns timescale) resolution enhancement can be achieved at low STED laser light levels. We will use cultured endothelial (HUVEC) cells in well-controlled in vitro models compatible with tau-STED.
During your PhD research you will apply a variety of techniques, including (but not limited to) molecular biology (cloning), eukaryotic cell culture (culturing and isolating primary immune and endothelial cells, viral transduction methods), fluorescence imaging, optogenetics, superresolution microscopy/STED, and image analysis.
Tasks and responsibilities:
You are passionate about science and have a particular interest in fundamental cell biology with affinity for microscopy. You like a challenge and are motivated to work in an interdisciplinary environment. You are a team player with good communication and problem-solving skills
Your experience and profile
You have/are:
It will work to your advantage if, in addition, you have experience with one or more of the following: viral transductions, proficiency with image analysis (e.g. FIJI, CellPose), and/or programming (e.g. R, Python, Matlab).
We offer a temporary contract for 38 hours per week for the duration of 4 years (the initial contract will be for a period of 18 months and after satisfactory evaluation it will be extended for a total duration of 4 years). The preferred starting date is 1 November 2025 or sooner. This should lead to a dissertation (PhD thesis). We will draft an educational plan that includes attendance of courses and (international) meetings. We also expect you to assist in teaching undergraduates and master students.
Based on a full-time appointment (38 hours per week) the gross monthly salary will range from €2.901 in the first year to €3.707 (scale P) in the last year. This does not include 8% holiday allowance and 8,3% year-end allowance. The UFO profile PhD candidate applicable The Collective Labour Agreement of Universities of the Netherlands is applicable.
Besides the salary and a vibrant and challenging environment at Science Park we offer you multiple fringe benefits:
Are you curious to read more about our extensive package of secondary employment benefits, take a look here.
The central aim for this project is to understand the molecular and cellular control of the barrier function of endothelial cells. In forming a monolayer, these cells partially overlap and form a tight barrier resisting sheer force and blood pressure. At the molecular scale, vascular endothelial (VE) cadherin and other proteins form a junctional protein complex (adherence junction), which tightly holds plasma membranes together of adjacent endothelial cells in a proximity of 20 nm. Intracellularly, these cell junctions are connected to the actin cytoskeleton via (α,β and p120) catenin. A variety of signalling cascades are connected to the regulation of the barrier integrity and cytoskeletal remodelling (including VEGFR and small GTPases).
This project focuses on the dynamics of the adherence junction and the connected cytoskeleton in relation to (local) pertubation by optogenetics, drugs and leukocyte transendothelial migration using super resolution microscopy. We will use a novel tau-STED microscope, which can achieve 50-70 nm optical resolution in live cells. By exploiting time-resolved information (ns timescale) resolution enhancement can be achieved at low STED laser light levels. We will use cultured endothelial (HUVEC) cells in well-controlled in vitro models compatible with tau-STED.
During your PhD research you will apply a variety of techniques, including (but not limited to) molecular biology (cloning), eukaryotic cell culture (culturing and isolating primary immune and endothelial cells, viral transduction methods), fluorescence imaging, optogenetics, superresolution microscopy/STED, and image analysis.
Tasks and responsibilities:
You are passionate about science and have a particular interest in fundamental cell biology with affinity for microscopy. You like a challenge and are motivated to work in an interdisciplinary environment. You are a team player with good communication and problem-solving skills
Your experience and profile
You have/are:
It will work to your advantage if, in addition, you have experience with one or more of the following: viral transductions, proficiency with image analysis (e.g. FIJI, CellPose), and/or programming (e.g. R, Python, Matlab).
We offer a temporary contract for 38 hours per week for the duration of 4 years (the initial contract will be for a period of 18 months and after satisfactory evaluation it will be extended for a total duration of 4 years). The preferred starting date is 1 November 2025 or sooner. This should lead to a dissertation (PhD thesis). We will draft an educational plan that includes attendance of courses and (international) meetings. We also expect you to assist in teaching undergraduates and master students.
Based on a full-time appointment (38 hours per week) the gross monthly salary will range from €2.901 in the first year to €3.707 (scale P) in the last year. This does not include 8% holiday allowance and 8,3% year-end allowance. The UFO profile PhD candidate applicable The Collective Labour Agreement of Universities of the Netherlands is applicable.
Besides the salary and a vibrant and challenging environment at Science Park we offer you multiple fringe benefits:
Are you curious to read more about our extensive package of secondary employment benefits, take a look here.
The Swammerdam Institute for Life Sciences (SILS) is located at the vibrant Amsterdam Science Park. SILS is one of eight institutes of the University of Amsterdam's Faculty of Science (FNWI). With around 240 employees, SILS carries out internationally high-quality life science research and provides education within various university programs. Research is also carried out in close cooperation with the medical, biotech, chemical, flavor, food & agricultural, and high-tech industries, and revolves around 4 main themes, Cell & Systems biology, Neurosciences, Microbiology and Green Life Sciences.
The Molecular Cytology research group studies the dynamic architecture of cells. Using genetically encoded fluorescent biosensors, we analyse processes at the molecular level in living mammalian cells. Our aim is to understand how cells respond to internal and external signals using advanced microscopy techniques.
The Swammerdam Institute for Life Sciences (SILS) is located at the vibrant Amsterdam Science Park. SILS is one of eight institutes of the University of Amsterdam's Faculty of Science (FNWI). With around 240 employees, SILS carries out internationally high-quality life science research and provides education within various university programs. Research is also carried out in close cooperation with the medical, biotech, chemical, flavor, food & agricultural, and high-tech industries, and revolves around 4 main themes, Cell & Systems biology, Neurosciences, Microbiology and Green Life Sciences.
The Molecular Cytology research group studies the dynamic architecture of cells. Using genetically encoded fluorescent biosensors, we analyse processes at the molecular level in living mammalian cells. Our aim is to understand how cells respond to internal and external signals using advanced microscopy techniques.
If you feel the profile fits you, and you are interested in the job, we look forward to receiving your application. We accept applications until and including 17 August 2025.
Applications should include the following information (all files besides your cv should be submitted in one single pdf file):
A knowledge security check can be part of the selection procedure. (for details: national knowledge security guidelines). Only complete applications received within the response period via the link below will be considered. The interviews will be held in the course of September 2025.
Do you have any questions or do you require additional information? Please contact:
If you feel the profile fits you, and you are interested in the job, we look forward to receiving your application. We accept applications until and including 17 August 2025.
Applications should include the following information (all files besides your cv should be submitted in one single pdf file):
A knowledge security check can be part of the selection procedure. (for details: national knowledge security guidelines). Only complete applications received within the response period via the link below will be considered. The interviews will be held in the course of September 2025.
Do you have any questions or do you require additional information? Please contact:
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