You will identify the E3 ubiquitin ligases and deubiquitinating enzymes (DUBs) that control MCL1 and NOXA turnover in dopaminergic neurons, and determine what happens to neuronal survival when you manipulate them. 
The backbone of the project is interaction proteomics: you will perform (co-) immunoprecipitations from neuronal cell models and brain tissue followed by quantitative LC-MS/MS, and you will run that workflow from end to end – from sample preparation to data analysis and interpretation. Candidate regulators are then validated biochemically, in cellular and in vivo models of PD, and finally in human post-mortem midbrain tissue, so that you can connect a molecular mechanism to the disease itself.
You will work in a group that combines molecular neuroscience with proteomics and neuropathology, and you will have the room to shape the direction of your own project.
Tasks and responsibilities:

• complete and defend a PhD thesis within the official appointment duration of four years;
• contribute to the design of experiments, perform them, and analyse and interpret the results, with a focus on (co-)immunoprecipitation and quantitative mass spectrometry;
• perform in vivo and ex vivo rodent experiments within the scope of your animal licence and the approved project licence;
• keep accurate records by thoroughly documenting and organising your work;
• take a leading role in writing manuscripts and present your results at (inter)national scientific meetings;
• participate in the Faculty of Science PhD training programme;
• assist in teaching and co-supervise junior scientists (technicians, BSc/MSc students).

You enjoy hands-on experimental work, and you keep going when an experiment fails: you look for the reason rather than repeating it unchanged. You plan, so that long workflows such as a proteomics experiment are prepared well before the instrument time starts. You are precise in documenting what you do. You discuss your data critically in lab meetings, you ask for help when you need it, and you are a nice colleague in an interdisciplinary team of scientists.

Your experience and profile:

• MSc in molecular biology, biochemistry, neurobiology or a related field;
• hands-on experience with mammalian cell culture, western blotting and (co-) immunoprecipitation;
• experience with the complete mass spectrometry workflow, from sample preparation and digestion through LC-MS/MS acquisition to data analysis and interpretation;
• a laboratory animal science certification (Article 9, rat/mouse) that is valid in the Netherlands at the start of the appointment;
• good knowledge of signal transduction and neuronal cell biology;
• good project and time management skills, enabling you to meet the project's deadlines for analyses, reports and other deliverables;
• good analytical, writing and presenting skills;
• fluency in English, both written and spoken, and the willingness to learn Dutch (in case you are not a Dutch native speaker).

A temporary contract for 38 hours per week for the duration of 4 years (the initial contract will be for a period of 18 months and after satisfactory evaluation it will be extended for a total duration of 4 years). The preferred starting date is as soon as possible. This should lead to a dissertation (PhD thesis). We will draft an educational plan that includes attendance of courses and (international) meetings. We also expect you to assist in teaching undergraduates and master students.
The gross monthly salary, based on 38 hours per week, ranges from € 3,204 in the first year to € 4,051 in the fourth year of employment (scale P). This does not include 8% holiday allowance and 8.3% year-end allowance. The UFO profile Promovendus is applicable. A favourable tax agreement, the 30% ruling, may apply to non-Dutch applicants. The Collective Labour Agreement of Universities of the Netherlands is applicable.

Curious about our extensive secondary benefits package? You can read more about it here.